Clitic climbing and restructuring in the history of French

This paper constitutes an empirical investigation into the diachrony of clitic climbing (and consequently restructuring) in French based on data from a novel corpus of legal texts, as well as a theoretical analysis of the loss of this phenomenon from the history of French. We show that clitic climbing was obligatory until the Middle French period, until its eventual loss before the start of the 19th century. Assuming that clitics are φ-heads that AGREE with v, we take restructuring to be monoclausal, despite apparent counterevidence where a subordinator or a Wh-item intervenes. We propose that restructuring is a necessary but not sufficient condition to clitic climbing, the latter depending on whether the upper v-head bears a set of unvalued φ-features. We associate the loss of clitic climbing to the loss of interpolation (i.e. the order [clitic-XP-V]), as we show that both constructions are available when cliticisation is a phonological mechanism only. In Modern French however, clitics are necessarily proclitic and verb-adjacent which indicates that cliticisation is syntactic. Lastly, we propose that French never lost restructuring, but instead it lost most transparency effects associated with it (such as clitic climbing), while retaining others (such as long passives and quantifier climbing)

Infinitive fronting as a transparency effect in Old and Middle French

In this article, we present a novel analysis of infinitive fronting in Old and Middle French (9th-16th century). We find that in sentences with modal verbs and clitic climbing, the infinitive may either follow the main verb or precede it. When the subject of the main verb is overt and the infinitive is fronted, the order is SUBJ-VINF-VFIN. Moreover, we find that the object of the fronted infinitive either cliticises onto the main verb (i.e. clitic climbing) or moves as a full DP with the infinitive, in which case the order is SUBJ-OBJ-VINF-VFIN. We compare our data to Stylistic Fronting, and we show that infinitive fronting in Old and Middle French is a different mechanism. Our analysis takes infinitive fronting to be vP-movement to Spec,TP, an operation which patterns alongside other Transparency Effects. Therefore, infinitive fronting provides further evidence for monoclausal restructuring in earlier French

A new gene involved in coenzyme Q biosynthesis in Escherichia coli: UbiI functions in aerobic C5-hydroxylation

International audienceCoenzyme Q (ubiquinone or Q) is a redox-active lipid found in organisms ranging from bacteria to mammals in which it plays a crucial role in energy-generating processes. Q biosynthesis is a complex pathway that involves multiple proteins. In this work, we show that the uncharacterized conserved visC gene is involved in Q biosynthesis in Escherichia coli, and we have renamed it ubiI. Based on genetic and biochemical experiments, we establish that the UbiI protein functions in the C5-hydroxylation reaction. A strain deficient in ubiI has a low level of Q and accumulates a compound derived from the Q biosynthetic pathway, which we purified and characterized. We also demonstrate that UbiI is only implicated in aerobic Q biosynthesis and that an alternative enzyme catalyzes the C5-hydroxylation reaction in the absence of oxygen. We have solved the crystal structure of a truncated form of UbiI. This structure shares many features with the canonical FAD-dependent para-hydroxybenzoate hydroxylase and represents the first structural characterization of a monooxygenase involved in Q biosynthesis. Site-directed mutagenesis confirms that residues of the flavin binding pocket of UbiI are important for activity. With our identification of UbiI, the three monooxygenases necessary for aerobic Q biosynthesis in E. coli are known

Legionella pneumophila: The Paradox of a Highly Sensitive Opportunistic Waterborne Pathogen Able to Persist in the Environment

International audienceLegionella pneumophila, the major causative agent of Legionnaires' disease, is found in freshwater environments in close association with free-living amoebae and multispecies biofilms, leading to persistence, spread, biocide resistance, and elevated virulence of the bacterium. Indeed, legionellosis outbreaks are mainly due to the ability of this bacterium to colonize and persist in water facilities, despite harsh physical and chemical treatments. However, these treatments are not totally efficient and, after a lag period, L. pneumophila may be able to quickly re-colonize these systems. Several natural compounds (biosurfactants, antimicrobial peptides…) with anti-Legionella properties have recently been described in the literature, highlighting their specific activities against this pathogen. In this review, we first consider this hallmark of Legionella to resist killing, in regard to its biofilm or host-associated life style. Then, we focus more accurately on natural anti-Legionella molecules described so far, which could provide new eco-friendly and alternative ways to struggle against this important pathogen in plumbing

In vitro identification of imidazo[1,2-a]pyrazine-based antileishmanial agents and evaluation of L. major casein kinase 1 inhibition

International audienceLeishmaniasis constitutes a severe public health problem, with an estimated prevalence of 12 million cases. This potentially fatal disease has a worldwide distribution and in 2012, the fatal Visceral Leishmaniasis (VL) was declared as new emerging disease in Europe, mainly due to global warming, with expected important public health impact. The available treatments are toxic, costly or lead to parasite resistance, thus there is an urgent need for new drugs with new mechanism of action. Previously, we reported the discovery of CTN1122, a potent imidazo[1,2-a]pyrazine-based antileishmanial hit compound targeting L-CK1.2 at low micromolar ranges. Here, we described structurally related, safe and selective compounds endowed with antiparasitic properties, better than miltefosine, the reference therapy by oral route. L-CK1.2 homology model gave the first structural explanations of the role of 4-pyridyl (CTN1122) and 2-aminopyrimidin-4-yl (compound 21) moieties, at the position 3 of the central core, in the low micromolar to nanomolar L-CK1.2 inhibition, whereas N-methylpyrazole derivative 11 remained inactive against the parasite kinase

Prognostic impact of the 2016 WHO classification of diffuse gliomas in the French POLA cohort

CERVOXY COLLInternational audienc